Subject: Chemistry Chapter: Chromatography Techniques
Topics: Thin Layer Chromatography (TLC)
Chromatography
This is a modern method that we can use for the separation of mixtures into its components, purification of compounds and also test the purity of compounds. The name chromatography comes from the Greek word ‘chroma’ meaning colour and ‘graphy’ for writing because the method was first used for the separation of coloured substances found in plants. This method was described by Tswett in 1906.
Principle of Chromatography
The technique of chromatography uses the difference in the rates at which the components of a mixture move through a porous medium (stationary phase) under the influence of some solvent or gas (moving phase).
Thus, this technique consists of two phases- one is a stationary phase of the large surface area while the second is a moving phase which is allowed to move slowly over the stationary phase. The stationary phase is either a solid or a liquid while the moving phase may be a liquid or a gas. There are also some other methods of purification like differential extraction and other chemical methods.
Thin-layer chromatography is a technique that is followed to separate different components present in a mixture. Let us learn more about it here.
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